Shanghai Chemtron Biotech Co.Ltd.

快乐飞艇杀一码的网址: Rapid Kit For HCV Nucleic Acid Detection And Its Detection Method 1

Chemtron biotech's present invention discloses a kit for rapid step detection of HCV nucleic acid, including nucleic acid extract, rt-qpcr reaction fluid, negative control, positive control and positive reference material. The present invention mainly solves the longtime disadvantage of testing nucleic acid of existing kits on the market. The hcv rapid test kit, based on the basic reaction principle of conventional rt-qpcr, realizes nucleic acid denaturation at a lower temperature by changing the composition of the reaction buffer and the primer, the unique design and synthesis of the probe, thus shortening the time difference of denaturation and temperature-restoring difference of PCR, and greatly shortening the whole reaction time. The kit of the present invention has high detection sensitivity, specificity and repeatability, and can detect six common genotypes of hepatitis c virus. Meanwhile, it is also suitable for regular quantitative PCR instrument, which provides an accurate basis for the auxiliary diagnosis of hepatitis c virus infection and the monitoring of drug treatment of its infected patients.

Principal claims:
1. HCV rapid test kit, which includes: nucleic acid extract, rt-qpcr reaction, enzyme promoting mixture, negative control, positive control and positive control products. Its features are: RT - qPCR reaction mixture including buffer, a pair of detection of hepatitis c virus nucleic acids and specific primers, a detection specificity of hepatitis c virus nucleic acid probe, a pair of globin pair specificity of detection, an induction gtobin specific probe which the reaction buffer containing the final concentration of 1-5% of the final concentration denaturant and enzyme denatured nucleic acid reagent, chemical modification of the bases for 0.1 0.5 mM buffer.

2. According to the rapid detection method of hepatitis c virus (HCV) nucleic acid in the kit described in claim 1, its characteristics are as follows: double-stranded DNA is a denatured enzymatic nucleic acid denaturator, which tends to break the hydrogen bond of the denaturator; Chemically modified bases can be altered, the agents of hybridization kinetics are primers or probes, and the stability of chemically modified nucleotides in the synthesis of double-stranded DNA can be changed.

3. The right to claim 1 or 2 through rapid detection of HCV nucleic acid kits is characterized by: the enzymatic denaturation of the denaturant is one or more of strong acid, strong base, monoalcohol, urea, AgCl, DMSO, betaine and formamide; 5-nitroindole reagent is chemically modified alkali (5-nitroindole), deoxymyoside, 2-amino-deoxy adenosine (2-amino-da), dcoxyribonuclcosidcs (superdntps), LNA or peptide nucleic acid (PNA) one or more.

4. Rapid detection of hepatitis c virus (HCV) nucleic acid according to the kit of claim 1 is characterized by: 200mM (PH8.0) tris-hcl, 500mMKCl, 0.8% (v/v) ethylphenyl polyethylene glycol as buffer (np-40).
Related News
  • How to Use OTC Ovulation Test Paper?

    How to Use OTC Ovulation Test Paper?

    April 22, 2019Ovary is an important organ of female reproductive system. Its main function is to produce and expel eggs and secrete hormones. It not only plays a decisive role in reproduction, but also is the sourc...view
  • What Is OTC?

    What Is OTC?

    July 26, 2018Otc is the abbreviation of over the counter, i.e. over-the-counter, which means that the consumer can purchase medication directly in the pharmacy or drug store without the need for a doctor prescript...view
  • Method of Detecting Treponema Pallidum and Rapid Strep Test Kit

    Method of Detecting Treponema Pallidum and Rapid Strep Test Kit

    April 9, 2019The detection of Treponema pallidum involves medical immunoassay methods, in particular the use of new syphilis-specific antigens. Immunological methods and rapid strep test kits can be used to detect...view
  • Diagnosis And Treatment Of Chlamydia

    Diagnosis And Treatment Of Chlamydia

    August 8, 2018The diagnosisdiagnosis1. Chlamydia pneumoniae infection has no specific manifestations in clinical and X-ray examinations. In particular, relevant etiology or serology examinations should be conducted...view
  • Urinary Microalbumin and Diabetes Mellitus

    Urinary Microalbumin and Diabetes Mellitus

    August 8, 2018Diabetes deaths are mostly due to diabetes complications. The increase of MUA in diabetic patients has been confirmed by a large number of clinical studies. In the past decade, it has been clearly rec...view
  • Product features and precautions of buy FSH test

    Product features and precautions of buy FSH test

    April 9, 20191. The product features of buy FSH test detection reagent of kaichuang biological(1) Kaichuang, a well-known brand of excellent birth and pregnancy, has a solid technical foundation(2) Adopt card type...view
  • TEL:+86-21-68129666
  • FAX:+86-21-68129222
  • EMAIL:[email protected]
  • ADDRESS:No.518, Qingdai Rd., International Medical Park, Pudong, 201318, Shanghai, P.R.China